Device

Part:BBa_K3058002:Design

Designed by: Ching Yuet To   Group: iGEM19_Hong_Kong_LFC_PC   (2019-10-10)


Uric acid-induced Uricase generator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2832
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2362
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2832
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2832
    Illegal NgoMIV site found at 867
    Illegal AgeI site found at 156
    Illegal AgeI site found at 268
    Illegal AgeI site found at 2384
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3498


Design Notes

To avoid codon-bias, the yeast HuR and URI gene is codon-optimized for better protein expression in E. coli.


Source

Sequence of HuR and URI gene in Cyberlindnera jadinii was codon-optimized by IDT's software and synthesized by IDT.

References